High-performance liquid chromatography

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high-performance liquid chromatography

(Abbreviated HPLC; also called high-pressure liquid chromatography.) Chromatographic technique similar to gas chromatography, except that various individual solvents, combinations of solvents, and changing concentrations of solvent are used in combination with stationary column phases to separate components of a liquid matrix.

Various detectors are used in HPLC, including UV (fixed and variable wavelength), refractive index, and conductivity detectors.


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